FastQCFastQC Report
Mon 6 Jun 2016
ERR1391377.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391377.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences984132
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA850.034.39233818
ATCGGAA952.3646862E-1128.40500619
AGATCGG1058.54925E-1125.69846317
CGTATGC709.3553354E-725.69846344
GATTGCG500.002213421522.5882974
TATCTCG702.7153616E-522.48615539
TCTCGTA702.7153616E-522.48615541
GCGGTTC1257.585186E-1021.5867128
CTCGTAT754.6236462E-520.98707642
CGGTTGT754.6236462E-520.98707632
CCGGTTG754.6236462E-520.98707631
AGCGGTT1301.2369128E-920.7564527
CGGAAGA1351.9772415E-919.98870821
TCGGAAG1351.9772415E-919.98870820
GAGCGGT1351.9772415E-919.98769226
ATCTCGT807.5974414E-519.67538540
CGCGATC807.5974414E-519.67538515
GCGCGAT707.011471E-419.27384814
CGAGCAG600.006531102618.73941410
CGGTTCA1507.3159754E-917.98892229
Produced by FastQC (version 0.11.5)