FastQCFastQC Report
Mon 6 Jun 2016
ERR1391376.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391376.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916686
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG351.2170676E-744.9740931
GACGATC250.002070205336.1290471
ATCGTTA458.7366425E-734.97984734
CGATCGT458.7366425E-734.97984732
GACCGAT501.9899635E-631.48186329
AGACCGA501.9899635E-631.48186328
ACCGATC554.1824333E-628.61987730
CGTTATC554.1824333E-628.61987736
AGCGACA450.001230489624.98560511
CTCGTAT651.5294765E-524.2181442
GAGCGGT702.6285352E-522.5806546
GCGGTTC702.7108408E-522.4907258
TCTCGTA702.713127E-522.48827241
TCGTATG702.713127E-522.48827243
CGGAAGA854.876816E-621.252381
TATCTCG855.05553E-621.16427639
CGGTTCA754.6198504E-520.9890549
CGTTGGA1301.1732482E-920.8436815
AGAGCGG807.355717E-519.7580725
CGTATGC807.5912205E-519.67723844
Produced by FastQC (version 0.11.5)