FastQCFastQC Report
Mon 6 Jun 2016
ERR1391370.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391370.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971690
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA504.901267E-835.98019841
CTCGTAT702.9007424E-828.91265942
GATCGGA1800.028.73566618
ATCGGAA1750.028.2716119
AGATCGG1850.026.74066217
CGTATGC709.3498056E-725.70014244
TCGGAAG2000.024.73765820
TCGTATG751.7053408E-623.986843
CCGAGAC2450.023.86441845
CCGTTGC903.303321E-722.48762531
GACCGTT955.55925E-721.30406429
ATCTCGT855.0549206E-621.16482240
ACCGTTG855.0549206E-621.16482230
AGCGGTT2550.021.16373327
CGGAAGA2350.021.05332621
CCGCGCC754.619409E-520.9895310
GCGGTTC2600.020.75780728
GGCGACA654.253344E-420.75780729
AGAGCGG2600.020.75673725
AAGAGCG2650.020.36719724
Produced by FastQC (version 0.11.5)