FastQCFastQC Report
Mon 6 Jun 2016
ERR1391369.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391369.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874730
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCC250.00211205635.98184610
CCGATGC453.5005694E-529.98658631
TCGTATA957.4578566E-1026.0394949
ATCGGAA802.98349E-622.49122619
GATCGGA702.7101936E-522.49122418
CTCGTAT500.00227007222.48993942
GACCGAT500.00227007222.48993929
ACCGATG500.00227007222.48993930
TCGTATG500.00227007222.48993943
CGATGCT602.4743335E-422.48993732
CCTTCGT1109.667383E-820.5157661
TCTTCGT1109.667383E-820.5157666
TATCTCG550.003946005420.44539839
AACGGGT550.00394731220.44423112
AGATCGG807.589723E-519.67757217
CTTCGTA1151.602748E-719.5553517
CGTATAA1051.4517573E-619.2759910
ATCTTCG1302.6766429E-819.0954445
ATCTCGT600.006526109818.74161540
TCTCGTA600.006526109818.74161541
Produced by FastQC (version 0.11.5)