FastQCFastQC Report
Mon 6 Jun 2016
ERR1391364.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391364.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875982
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGGG300.00515999429.98405520
GATCGGA1350.028.31827418
GGGCACG607.945968E-626.3518833
CTCGTAT608.226158E-626.2345542
AGATCGG1500.025.48353617
CCTTCGT901.142871E-825.095591
GACGAGG450.00123053824.98528544
TCGGAAG1550.024.664320
ATCGGAA1550.024.664319
CGTATGC751.7055936E-623.98587444
TCTCGTA702.7143604E-522.48675741
ACTGCGC955.563088E-721.3020279
TCGTATG754.6219488E-520.98763843
GAGACGA654.2539887E-420.75700842
GGGCGAC654.2539887E-420.75700828
GGCGCCC550.003947939720.44367223
AGCGGTT2000.020.23692527
TCGTTGG1351.869921E-920.0776274
CCGAGAC1800.019.98822845
TCGTATA807.597997E-519.6747889
Produced by FastQC (version 0.11.5)