FastQCFastQC Report
Mon 6 Jun 2016
ERR1391362.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391362.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970819
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA751.713488E-929.98375918
CCGCTGA554.179972E-628.62233531
AGATCGG803.414243E-928.10977417
ATCGGAA856.51562E-926.45625719
TCGGAAG901.1974407E-824.98646520
CGCTGAT551.372546E-424.5334332
ACCGCTG651.5292258E-524.218930
CTCGTAT654.2530906E-420.75798842
CTTCGTA1009.0946014E-720.240087
CGTCCTC600.006528224318.74081430
ACGATGT600.006530165718.7398517
TCGTATA851.20872835E-418.5203349
GAGCGGT1253.8514554E-717.99118226
AGCGGTT1305.810034E-717.29921527
GCGGTTC1459.7170414E-817.06060428
CGTATGC800.001716795316.86586444
TCTCGTA800.001716795316.86586441
AGAGCGG1501.4300167E-716.49191925
TTCGTAT1104.655814E-516.355628
GACCGCT850.002573178415.87457229
Produced by FastQC (version 0.11.5)