FastQCFastQC Report
Mon 6 Jun 2016
ERR1391359.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391359.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537331
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGG401.5182266E-533.880961
CGTATGC551.1308657E-732.71055244
CGGTCAT551.1317934E-732.70750832
ACCGGTC651.4088073E-831.13503330
GACCGGT651.4088073E-831.13503329
CTTCGGT300.00505063130.1135922
TTCGGTG300.00505063130.1135923
CTCGTAT602.4277324E-729.98188442
TCGTATG602.4277324E-729.98188443
CCGGTCA602.4277324E-729.98188431
TCTCGTA751.7037728E-623.9855141
AGACCGG751.7037728E-623.9855128
GTATGCC802.9830662E-622.48850645
GATCGGA602.4752354E-422.48641418
CCGAGAC802.9854782E-622.48641425
ATCGGAA654.2514794E-420.7566919
ATCTCGT951.3205612E-518.93592840
GAGACCG951.3205612E-518.93592827
TATCTCG951.3205612E-518.93592839
GCCGGGT600.006528815318.7386788
Produced by FastQC (version 0.11.5)