FastQCFastQC Report
Mon 6 Jun 2016
ERR1391353.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391353.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820717
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC10500.1279369136986318Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG850.039.68776332
AGATCGG1900.037.8751417
GATCGGA1950.036.90622718
TCGGAAG1950.036.90622720
ATCGGAA2000.035.98357419
CCGTTAC950.035.51010531
GACCGTT1000.033.73665629
ACCGTTA1000.033.73665630
CGTATGC1000.033.734644
TCTCGTA1000.033.734641
TTACGTA1050.032.1281934
TACGTAT1050.032.1281935
ATCTCGT1150.029.33443540
GTTACGT1150.029.33443533
CGAGATC2500.028.78510715
TCGTATG1350.028.32040443
TATCTCG1200.028.11216739
CGGAAGA2700.027.48745221
AGACCGT1250.026.98932528
ACGTATC1250.026.98768236
Produced by FastQC (version 0.11.5)