FastQCFastQC Report
Mon 6 Jun 2016
ERR1391352.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391352.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744764
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA651.4089892E-831.13841641
AGACCGC651.4089892E-831.13841628
AGATCGG952.3646862E-1128.40697517
CGTATGC755.657057E-826.9884444
CGCTTAG755.660695E-826.98662832
GATCGGA1004.5474735E-1126.98662618
ATCGGAA1004.5474735E-1126.98662619
TCGGAAG1058.54925E-1125.7015520
CCGCTTA801.0578151E-725.29996331
TATCTCG801.0578151E-725.29996339
CTCGTAT851.9008621E-723.81172842
GACCGCT851.9008621E-723.81172829
TCGTATG903.2994467E-722.48885543
GTATGCC955.5491546E-721.30666245
ACCGCTT1009.0892536E-720.2399730
GAGACCG1009.0892536E-720.2399727
CCGAGAC1503.45608E-1019.4916545
ATCTCGT1051.4510406E-619.27616340
GCGGTTC1454.793037E-918.61146528
CGGAAGA1608.203642E-1018.27219621
Produced by FastQC (version 0.11.5)