FastQCFastQC Report
Mon 6 Jun 2016
ERR1391349.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391349.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences939544
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG554.177313E-628.6245143
TCTCGTA608.215835E-626.23913441
CGTATGC651.528259E-524.2207444
GAGACCG802.9845014E-622.49068627
CCGAACT702.710971E-522.49068631
TCGTATA1204.5474735E-1022.4894899
ACCGAAC855.048667E-621.16770430
CTCGTAT754.6161862E-520.99130842
ATCTTCG1301.1841621E-920.827145
CTTCGTA1503.45608E-1019.490897
GATCGGA707.0016505E-419.2777318
AGATCGG707.004159E-419.27670517
CGTATAA1403.103196E-919.27670510
CCGCACC600.006527215218.7412410
TTCGTAT1454.796675E-918.611998
TCTTCGT1708.731149E-1118.5810786
ATCGGAA750.00111310617.9925519
ATCTCGT901.8711337E-417.49275640
GACCGAA901.8711337E-417.49275629
TATCTCG901.8711337E-417.49275639
Produced by FastQC (version 0.11.5)