FastQCFastQC Report
Mon 6 Jun 2016
ERR1391348.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391348.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences932257
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA1500.026.9901510
CTTCGTA1550.024.6684197
TCGTATA1550.024.6684199
TTCGTAT1800.022.4917958
ATCTTCG2000.021.4269685
TCGTATG550.00394431220.44708643
TTCGTTC550.00394553820.4459915
GATCGGA807.582271E-519.68031918
TCTTCGT2250.019.0461926
CTCGTAT600.006523316718.74316242
AGACCGC600.006523316718.74316228
TGGCGCG1002.053338E-517.9924712
GCGCGAT901.8711766E-417.4926814
GGCGCGA901.8711766E-417.4926813
TCGGAAG952.8264904E-416.572920
AGATCGG1004.1792679E-415.74341117
CGGTTCA1056.05207E-414.994539
GTGGCGC1402.2378925E-514.45823411
ATCGGAA1108.6099346E-414.3129619
CGCGATC1108.613431E-414.31219215
Produced by FastQC (version 0.11.5)