FastQCFastQC Report
Mon 6 Jun 2016
ERR1391345.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391345.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834719
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA702.1827873E-1135.33836418
AGATCGG801.0004442E-1030.92106817
ATCGGAA801.0004442E-1030.92106819
GCGGAGC300.00515984729.98406432
GAGCGGT1101.5279511E-1024.53241726
CGGAAGA1052.3792381E-923.55890821
TCGGAAG1052.3792381E-923.55890820
GCGGTTC1152.6739144E-1023.46579228
AGCGGTT1152.6739144E-1023.46579227
CGGTTCA1204.5474735E-1022.48804929
TCGATCT855.0526796E-621.16522411
ACGCCCA908.284756E-619.98937612
CCGAGAC1403.099558E-919.2777845
AAGAGCG1403.103196E-919.2754724
CGAGATC1555.366019E-1018.86094715
AGAGCGG1555.366019E-1018.86094725
CCGAGAT1454.798494E-918.610814
CTCGATC851.2088526E-418.5195710
ACGATCT750.001085055818.060762
CGTTGGA1406.229493E-817.737185
Produced by FastQC (version 0.11.5)