FastQCFastQC Report
Mon 6 Jun 2016
ERR1391344.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391344.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences777218
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA702.8987415E-828.91302118
AGATCGG856.511982E-926.4563617
AGCGCTC851.9006802E-723.8122567
ATCGGAA955.55563E-721.30433319
CGCCCGG754.6192676E-520.98871213
ACGCCCG807.590275E-519.67691812
ATCTTCG1051.3989848E-619.3500395
ACCACGC2250.018.9897869
GCGCTCC951.3203133E-518.9384028
CGGCTAA1354.2931788E-818.32347717
GCGGTTC1153.4613477E-617.60036328
GAGCGGT1153.4613477E-617.60036326
CCACGCC2053.6379788E-1217.55153710
CCGGCTA1305.809252E-717.2983916
AGCGGTT1205.192096E-616.86701427
AGAGCGG1257.654124E-616.19233525
TCGGAAG1257.658802E-616.19129220
GAAAGCG850.002507813515.9353274
TCGTATA850.00257337715.8738169
CTTCGTA1004.179899E-415.7425467
Produced by FastQC (version 0.11.5)