FastQCFastQC Report
Mon 6 Jun 2016
ERR1391343.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391343.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862534
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC602.4282963E-729.98515944
CGATTAC654.897083E-727.675432
AGATCGG903.9835868E-1027.48320617
GATCGGA903.9835868E-1027.48320618
AGACCGA709.352716E-725.69858428
GACCGAT751.7058701E-623.98534429
TCGTATG802.9861258E-622.48886943
ACGCCCG500.002272204522.48626112
CCGATTA802.9891344E-622.48626131
ATCGGAA1156.8102963E-921.50859619
CTCGTAT908.282672E-619.99010542
TCTCGTA908.282672E-619.99010541
TCGGAAG1251.778244E-819.78790920
CCGAGAC1503.45608E-1019.49035345
CGCCCGG600.006532218.73855213
CGCCACC2100.018.2847825
ACCGATT1002.0564486E-517.9890130
CATCTCG1002.0564486E-517.9890139
GCGGTTC1601.6239937E-816.86469728
CGAGATC1601.6239937E-816.86469715
Produced by FastQC (version 0.11.5)