FastQCFastQC Report
Mon 6 Jun 2016
ERR1391342.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391342.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789505
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1300.027.67753618
AGATCGG1450.024.81434217
TCGGAAG1500.023.98719820
CCGAGAC1650.021.80930745
ATCGGAA1800.021.23866719
CGGAAGA1750.020.56045521
GCGGTTC1653.6379788E-1220.44363428
GAGCGGT1653.6379788E-1220.44363426
AGCGGTT1703.6379788E-1219.84235227
AGAGCGG1757.2759576E-1219.27542725
CGGTTCA1851.6370905E-1118.23351329
AAGAGCG2159.094947E-1216.73525424
CCTTCGT1104.506414E-516.4172921
GAGATCG2201.2732926E-1116.35490816
GCGAGAC900.003765875714.99238
AGCGAGA900.003765875714.99237
ATGCCGA2457.094059E-1114.68789942
CGAGTAG1000.007383441613.5451275
CGTTGGA1352.5764652E-413.3779035
CTTCGTT1352.5778526E-413.3770522
Produced by FastQC (version 0.11.5)