FastQCFastQC Report
Mon 6 Jun 2016
ERR1391341.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391341.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913386
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1100.038.8413218
AGATCGG1150.037.1505317
ATCGGAA1200.035.60454619
GACCGTT855.456968E-1231.74472629
ACCGTTC901.2732926E-1129.9811330
CCGTTCA901.2732926E-1129.9811331
TCGGAAG1450.029.46582820
AGACCGT952.3646862E-1128.40317728
CTCGTAT1152.6739144E-1023.46349542
TCTCGTA1204.5474735E-1022.48584741
TCGTATG1204.5474735E-1022.48584743
CTTCGTA955.562724E-721.3023837
CCTTCGT1301.1659722E-920.8532031
TCGTTGG1850.020.7592684
GAGCGGT1850.020.6626726
TCCGACA550.00395029520.441689
CGTTGGA1900.020.2129735
AGCGGTT1900.020.11891727
GAGACCG1351.9772415E-919.98742127
CGTTCAC1403.108653E-919.27358632
Produced by FastQC (version 0.11.5)