FastQCFastQC Report
Mon 6 Jun 2016
ERR1391340.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391340.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853443
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA651.4091711E-831.13903841
GACCGCG300.005157022729.98749719
CTCGTAT654.89119E-727.67914642
CCGTTAA654.89621E-727.67590331
TCGGAAG1550.026.11814320
CGTATGC709.3414747E-725.70206644
GACCGTT709.3510243E-725.69905329
CGTTAAC709.3510243E-725.69905332
GATCGGA1650.024.53522518
ATCGGAA1650.024.53522519
AGATCGG1650.024.53091217
CATCTCG903.2999742E-722.48930539
TCGTATG855.050453E-621.16640743
CCGAGAC1900.020.1220145
CGGAAGA2050.019.74786621
ATCTCGT1051.4512698E-619.27654840
GCGGTTC2250.018.98874528
GGCACGA951.32192E-518.93614413
ACCGTTA951.32192E-518.93614430
GGCGACA750.00111432717.98933629
Produced by FastQC (version 0.11.5)