FastQCFastQC Report
Mon 6 Jun 2016
ERR1391336.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391336.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences781473
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA501.987437E-631.4859818
TATCTCG453.498519E-529.98856739
GACGCTT300.005155863229.98856442
ATCGGAA554.1771436E-628.62361519
AGATCGG608.227766E-626.23327817
CTGCGAA450.001230307324.98567433
TCGGAAG651.5281998E-524.21998420
CGTATGC702.7068507E-522.49430544
CTCGTAT702.7095291E-522.49142542
ATCTCGT654.2474706E-420.76131440
TCTCGTA807.581219E-519.67999641
AGACCGG707.0077094E-419.27466428
ACCGGCT750.001114067517.98968530
GACCGGC750.001114067517.98968529
CGGCTTA750.001114067517.98968532
CGCCCGG901.87382E-417.48885213
CGCCACC1551.0435542E-817.4787145
TCGTATG800.001714550516.86856843
GAGACCG800.001717489816.8642527
CGGGATT1205.2002742E-616.8642514
Produced by FastQC (version 0.11.5)