FastQCFastQC Report
Mon 6 Jun 2016
ERR1391333.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391333.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738194
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT406.1827374E-428.11043542
TCTCGTA507.180257E-526.9860241
CGGTTCT450.001229846524.98705516
TCGTATA851.9012259E-723.8111939
CGTATGC602.4738754E-422.48987244
GACCGTT500.002270671822.4883529
CCGTTGT500.002270671822.4883531
ATCTCGT654.2510833E-420.75847840
GCGGTTC654.2510833E-420.7584788
CGTATAA1101.00266334E-720.44395410
AAGACGG550.00394704620.4439548
CATCTCG550.00394704620.44395439
CTTCGTA1009.0909816E-720.2395157
AGCGGTT707.0046E-419.2757287
TCGTATG707.0046E-419.27572843
CGTTGTA600.006527830418.74029232
TTCGTAT1202.5072586E-718.7402928
TCGTTGG1506.9576345E-918.0604114
CGGTTCA750.001113575217.990689
CCTTCGT1406.223672E-817.7379041
Produced by FastQC (version 0.11.5)