FastQCFastQC Report
Mon 6 Jun 2016
ERR1391331.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391331.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences843886
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG801.0004442E-1030.91855817
AATTACG300.00516192829.9816314
TCGGAAG903.9835868E-1027.4847920
GATCGGA957.4578566E-1026.03822318
CTTCGTA957.4578566E-1026.036687
TCGTATA801.05936124E-725.2979
TCTCGTA450.001229820224.98765441
TTCGTAT901.1978045E-824.9846948
ATCGGAA1101.5279511E-1024.5318819
CGTATGC500.002270621722.4888944
CTCGTAT500.002270621722.4888942
ACCGCTC500.00227218622.48622530
ACCGCGC702.714705E-522.4862259
ACTCCCG500.00227218622.48622511
CGCTCTA602.4770017E-422.48622332
CGTATAA955.560796E-721.30273810
CACCGCG754.6225316E-520.9871438
ACGCCCG654.254491E-420.75651612
ATCTTCG1201.0571966E-820.7031465
TCCCGTA707.0101937E-419.2739079
Produced by FastQC (version 0.11.5)