FastQCFastQC Report
Mon 6 Jun 2016
ERR1391329.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391329.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849476
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC606.4574124E-933.73419628
GATCGGA1050.032.12780418
CGTATGC651.4082616E-831.1410944
ATCGGAA1150.029.33408419
TCTCGTA702.8958311E-828.91672941
TCGTATG702.8958311E-828.91672943
GACCGCT702.8974682E-828.91502629
TATCTCG702.8974682E-828.91502639
CTCGTAT755.6579665E-826.98894542
AGATCGG1250.026.98735417
TCGGAAG1250.026.98735420
ATCTCGT851.8999344E-723.81377640
ACCGCTT851.9010258E-723.81237430
GCGGTTC1500.022.48946428
GTATGCC1056.1296305E-821.41979845
CCGCTTG955.553193E-721.30580731
GAGACCG955.553193E-721.30580727
AGAGCGG1601.8189894E-1221.08387225
AGCGGTT1653.6379788E-1220.44496727
CCGAGAC1552.3646862E-1120.3142645
Produced by FastQC (version 0.11.5)