FastQCFastQC Report
Mon 6 Jun 2016
ERR1391327.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391327.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678453
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA451.9355866E-839.9781241
CGACTCT250.00206896736.13211
GATCGGA754.7293724E-1132.98194518
AGATCGG754.7293724E-1132.9795217
ATCGGAA801.0004442E-1030.92057619
TCGGAAG901.2732926E-1129.9835920
GACCGTC654.888625E-727.679229
CTCGTAT654.891772E-727.6771642
TCGTATG654.891772E-727.6771643
CTTCGTA608.224633E-626.2337067
CGTATGC709.3366E-725.70211244
ACCGTCT709.3366E-725.70211230
CGGCTAA1159.094947E-1225.41899317
ATCTCGT801.0580516E-725.29865340
TTCGTAT450.001230482224.9844828
CCGGCTA1201.6370905E-1124.3598716
CCGCCAC651.4808091E-524.3178844
GAGCGGT1156.7993824E-921.50996626
AGACCGT855.0478484E-621.16644728
CCCGCCA754.4735934E-521.0754973
Produced by FastQC (version 0.11.5)