FastQCFastQC Report
Mon 6 Jun 2016
ERR1391326.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391326.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883762
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA708.1854523E-1032.1243141
CTCGTAT751.713488E-929.98268742
TATCTCG803.414243E-928.1087739
GATCGGA903.965397E-1027.48879418
GACCGTT903.9835868E-1027.48724229
TCGTATG856.517439E-926.45531343
TCAGCGT450.001202322825.0849193
CGTATGC901.1961674E-824.98840144
CCGTTGC901.196895E-824.98698631
ATCTCGT901.1976226E-824.98557340
TCGGAAG1001.3496901E-924.73991620
AGATCGG1101.5279511E-1024.5312917
ACCGTTG952.1243977E-823.6732230
ATCGGAA1052.3756002E-923.56182519
CGTTGCA1056.137452E-821.41741832
AGACCGT1201.1103111E-820.6154328
GATCGCG600.00638312518.8136884
GAGCGGT1202.4076144E-718.8136886
AGCGGTT1406.513801E-817.668377
GAGACCG1305.8104524E-717.29868327
Produced by FastQC (version 0.11.5)