FastQCFastQC Report
Mon 6 Jun 2016
ERR1391324.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391324.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891491
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTAA751.8189894E-1235.980332
CCGCTTA754.7293724E-1132.98194531
AGACCGC852.0372681E-1029.10171528
CGTATGC1004.5474735E-1126.99128344
GACCGCT957.4578566E-1026.03837629
ATCTCGT1001.3515091E-924.73923340
ACCGCTT1052.3792381E-923.5585330
CTCGTAT1104.0690793E-922.49021142
TCTCGTA1104.0690793E-922.49021141
TCGTATG1104.0690793E-922.49021143
CGGTTCA1507.3068804E-917.990159
CGGAAGA1406.233313E-817.7365461
GTATGCC1406.499249E-817.67187145
GCGGTTC1406.5076165E-817.669898
CATCTCG1406.5076165E-817.6698939
GAGACCG1459.7206794E-817.05962827
CCGAGAC1459.7206794E-817.05962825
TCGTTGG1257.3849133E-616.2540424
GAGCGGT1551.9877734E-716.0210086
GCTTAAC1552.0773768E-715.95900533
Produced by FastQC (version 0.11.5)