FastQCFastQC Report
Mon 6 Jun 2016
ERR1391323.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391323.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences932394
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT501.0859367E-940.48001542
CGTATGC651.4097168E-831.13847444
TCTCGTA751.711669E-929.98519741
CGCCTAA755.6666977E-826.9852332
CCGCCTA801.0589429E-725.29865331
GATCGGA1251.8189894E-1225.18756518
TCGGAAG1251.8189894E-1225.18756520
ATCGGAA1301.8189894E-1224.21881319
AGATCGG1301.8189894E-1224.21751417
AGACCGC851.9028812E-723.81049728
TCGTATG903.3012293E-722.48889743
GACCGCC903.3029391E-722.48769229
TCGTATA955.558595E-721.3041299
ACCGCCT1009.098803E-720.23892230
ATCTCGT1102.2657132E-618.40000740
TATCTCG1102.2668737E-618.39902139
CCGAGAC1902.3646862E-1117.75439345
GAGACCG1153.4652876E-617.59906227
TTCGTAT1358.627321E-716.6575498
GTATGCC1257.65888E-616.19200745
Produced by FastQC (version 0.11.5)