FastQCFastQC Report
Mon 6 Jun 2016
ERR1391321.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391321.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences973256
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAA250.002113775435.97628412
TCTCGTA406.1855454E-428.10936241
GATCGGA654.8914444E-727.67975418
ATCGGAA709.3419476E-725.7026319
CTCGTAT450.001230402224.986142
TCGTATG500.002271692322.4874943
CCGGCTA1305.0931703E-1122.4851816
CGGCTAA1459.094947E-1221.7098317
GACCGTT550.00394646120.44527229
ACCGTTC550.00394646120.44527230
TAAGCGA550.00394646120.44527231
GCGGTTC908.281497E-619.99093428
AGATCGG908.2962215E-619.98682617
CGCCCGG1051.4542511E-619.2730113
ATCCGCC600.00652685718.74149933
TCGGAAG1002.0541076E-517.9918420
CCTTCGT1153.3290726E-617.6733671
AGACCGT800.001715787516.86735228
CCGTTCA800.001715787516.86735231
AGAGCGG1104.6625733E-516.35285625
Produced by FastQC (version 0.11.5)