FastQCFastQC Report
Mon 6 Jun 2016
ERR1391319.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391319.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903244
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT651.4109901E-831.1351842
TCGTATG702.9016519E-828.9112443
CGTATGC755.6692443E-826.98382444
GACCGAA856.522896E-926.45326429
TCTCGTA801.0593976E-725.29733541
GATCGGA1500.022.48651918
AGATCGG1500.022.48527517
ACCGAAG903.3061224E-722.48527330
CCGAAGT903.3061224E-722.48527331
CGAAGTT903.3061224E-722.48527332
AGACCGA955.563943E-721.3018428
ATCGGAA1601.8189894E-1221.08111219
ATCTCGT1009.102714E-720.23786740
TCGGAAG1703.6379788E-1219.84104720
ATCTTCG1950.019.6963185
AATCTCG1202.5124245E-718.7377339
CTTCGTA1801.0913936E-1118.7377287
GAGCGGT1851.6370905E-1118.23130426
GGGCGAC750.001114859517.9882228
GAGACCG1153.4686E-617.59717227
Produced by FastQC (version 0.11.5)