FastQCFastQC Report
Mon 6 Jun 2016
ERR1391317.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391317.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029280
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC40670.39513057671381935Illumina Paired End PCR Primer 2 (100% over 35bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAAGTAATCTCGTATGCC29350.2851507850147676Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG3350.039.6021843
CTCGTAT3500.038.547442
CGTATGC3400.038.3621744
CCGTAAG3750.036.5789831
TCTCGTA3800.034.9124541
ATCTCGT4100.033.45475840
GACCGTA4050.033.3141929
GATCGGA5550.033.2241518
CGTAAGT4100.032.90791732
AGACCGT4150.032.51143628
GTATGCC4050.032.2052845
AATCTCG4400.031.68479539
AGATCGG5900.031.63282817
TCGGAAG5800.031.40436720
ACCGTAA4300.031.3773230
ATCGGAA5850.031.13595419
GAGACCG4500.029.98276927
TATGCCG300.00516271529.98131643
AGCTCGT300.00516271529.98131643
AGTAATC5100.027.33723336
Produced by FastQC (version 0.11.5)