FastQCFastQC Report
Mon 6 Jun 2016
ERR1391315.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391315.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences995548
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAA651.4109901E-831.1360131
GATCGGA1200.026.2349718
TCGTATG801.05936124E-725.29800643
AGATCGG1350.024.98568717
CGTATGC851.903627E-723.80988944
TCTCGTA851.903627E-723.80988941
CGGTAAT851.903627E-723.80988932
GACCGGT955.5607416E-721.30358529
ATCTCGT1101.0041367E-720.44283340
TCGGAAG1552.5465852E-1120.31094620
ATCGGAA1552.5465852E-1120.31094619
CTCGTAT1009.102314E-720.23840542
ACCGGTA1051.4531197E-619.27467230
GAGCGGT1902.3646862E-1117.75298726
AGACCGG1153.46661E-617.59861428
AATCTCG1153.46661E-617.59861439
GCCACCG1257.3603624E-616.2601436
GCGGTTC1956.530172E-1016.14459628
GAATCGC1754.8950824E-815.41973812
AGCGGTT2051.3260433E-915.35705627
Produced by FastQC (version 0.11.5)