FastQCFastQC Report
Mon 6 Jun 2016
ERR1391314.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391314.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences648455
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA29460.4543106306528595No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA22160.3417353555759459No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAAGAATCTCGTATGCCGTC21360.32939833912916083Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAAGAATCTCGTATGCC20180.3112012398701529Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT10340.15945593757469678Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATGA2900.043.43904517
TAAGCCG2900.043.43904513
CGTCATA2900.043.43904524
TATGCCG2550.043.2261143
GCCGATG3000.041.99107716
AAGCCGA3000.041.99107714
ATGCCGT2650.041.59493644
AGCCGAT3050.041.302715
ACGTCAT3050.041.302723
GGCGGTC2950.041.17769235
GACGTCA3100.040.63652422
AGGCGGT3000.040.49139834
GATGACG3200.040.0696119
CGATGAC3150.039.991518
TTCGGTA3100.039.91087322
TCGGTAT3050.039.82760223
TGACGTC3200.039.36663421
GCGGTCT3100.039.1852236
ATTCGGT3100.039.1852221
AATTCGG3100.039.1852220
Produced by FastQC (version 0.11.5)