FastQCFastQC Report
Mon 6 Jun 2016
ERR1391312.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391312.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681607
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA35040.5140792274727225No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA27360.401404328300619No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATAACAATCTCGTATGCCGTC15280.22417610147783107Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATAACAATCTCGTATGCC14030.20583708794070485Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT7280.10680641484022317Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAT3900.043.83829515
CGATGAC3950.043.2897318
CGTCATA4000.042.74860424
GCCGATG4050.042.2146516
TTCGGTA3850.042.0765522
TCGGTAT3850.042.0765523
GACGTCA4100.041.7059622
CGGTATT3900.041.53710624
GCGGTCT3750.041.39257436
ACGTCAT4150.041.2034823
TAAGCCG4150.041.1974313
AAGCCGA4200.040.7069814
AATTCGG4000.040.49867620
TCGGGGA3850.040.31744414
GGCGGTC3850.040.31744435
ATGACGT4250.040.23398220
CCGATGA4250.040.2280817
TGCCGTC1800.040.01043745
ATGCCGT1800.039.9928344
TATGCCG1750.039.84999543