FastQCFastQC Report
Mon 6 Jun 2016
ERR1391311.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391311.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences733887
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA40790.5558076379606125No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA25710.3503264126493588No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAATTAGATCTCGTATGCCGTC15460.21065913417188203Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAATTAGATCTCGTATGCC14080.19185514936223153Illumina Paired End PCR Primer 2 (97% over 34bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT7990.10887234683268678Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTAT3800.043.2171423
TTCGGTA3850.042.6558822
GCGGTCT3900.042.1090136
GGCGGTC3900.042.1090135
GAATTCG3900.042.1090119
AATTCGG3950.041.57598520
TAAGCCG3950.041.57598513
CGATGAC4050.041.1048918
AGCCGAT4000.041.05628215
CGGTCTC4100.040.6036137
ATTCGGT4050.040.5494221
AGGCGGT4050.040.5494234
GATGACG4050.040.5494219
CGGGGAA4000.040.4938715
GACGTCA4100.040.05491322
CGTCATA4100.040.05491324
CGGTATT4200.039.63685624
GCCGATG4150.039.57232316
ACGTCAT4150.039.57232323
TGACGTC4200.039.10122321