FastQCFastQC Report
Mon 6 Jun 2016
ERR1391310.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391310.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720685
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA21420.297217230829003No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA17180.23838431492260834No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAAGTATATCTCGTATGCCGTC16260.2256186822259378Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAGTATATCTCGTATGCC12860.17844134399911196Illumina Paired End PCR Primer 2 (97% over 34bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT8170.11336436862151981Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATT2250.040.99829524
TTCGGTA2300.040.10424422
AGCCGAT1750.039.8527215
TAAGCCG1750.039.8527213
TCGGTAT2350.039.25368523
TCCTCGG2250.039.0119111
TGCCGTC1850.038.94161645
TATGCCG1800.038.74569743
CGGTCTC2400.038.435937
GAATTCG2400.038.4332319
TCGGGGA2500.037.79580314
CGTCATA1850.037.70113424
GCCGATG1850.037.69851716
GCGGTCT2500.036.8984636
GGCGGTC2500.036.8984635
AAGCCGA1900.036.7064514
CGGGGAA2550.036.17245515
AATTCGG2550.036.17245520
CGTGAAC250.002104866836.0059971
GATGACG1950.035.7652619
Produced by FastQC (version 0.11.5)