ERR1391309.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1391309.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	689100
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA	2981	0.4325932375562328	No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA	2231	0.3237556232767378	No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGACATAAATCTCGTATGCCGTC	1992	0.2890727035263387	Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACATAAATCTCGTATGCC	1705	0.24742417646205195	Illumina Paired End PCR Primer 2 (97% over 34bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT	899	0.13046002031635467	Illumina Paired End PCR Primer 2 (96% over 30bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
AGCCGAT	310	42.817265	15
TGACGTC	315	42.137627	21
CGTCATA	315	42.137627	24
TAAGCCG	320	41.47922	13
TCGGGGA	350	41.13775	14
ATGACGT	325	40.841084	20
GACGTCA	330	40.22228	22
GCCGATG	330	40.22228	16
AAGCCGA	330	40.22228	14
ACGTCAT	330	40.22228	23
GGCGGTC	350	39.852196	35
CTCGGGG	295	39.656094	13
AATTCGG	365	39.447155	20
TTCGGTA	365	39.447155	22
GCGGTCT	360	39.370113	36
TATGCCG	235	39.250446	43
GATGACG	340	39.03927	19
AGATTCG	70	38.58904	10
GATTCGG	70	38.58904	11
CGATGAC	345	38.473484	18