FastQCFastQC Report
Mon 6 Jun 2016
ERR1391308.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391308.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641982
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA24690.3845902221557614No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA17620.27446252387138576No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATTGAAATCTCGTATGCCGTC15750.24533398132657924Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATTGAAATCTCGTATGCC14560.22679763607079328Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT7980.12430255053880014Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTAT2100.044.9992223
ATTCGGT2150.043.95272421
TTCGGTA2150.043.95272422
TAAGCCG2950.041.9451613
GGCGGTC2200.041.9310935
AGCCGAT3050.041.30754515
CCGATGA3050.041.30754517
GCCGATG3050.041.30754516
AAGCCGA3050.041.30754514
GAATTCG2300.041.08304619
CGATGAC3100.040.64129318
GATGACG3050.040.56991219
TGCCGTC1950.040.3902145
TATGCCG1900.040.25932743
CGGTATT2350.040.21207424
AATTCGG2350.040.2089420
GCGGTCT2300.040.10836
AGGCGGT2300.040.10834
GACGTCA3050.039.83537722
TGACGTC3050.039.83537721
Produced by FastQC (version 0.11.5)