FastQCFastQC Report
Mon 6 Jun 2016
ERR1391305.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391305.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724444
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA40500.5590494227297073No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA31840.4395094720917007No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATACAAATCTCGTATGCC19890.27455538316281175Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATACAAATCTCGTATGCCGTC19790.27317501421780016Illumina Paired End PCR Primer 2 (96% over 32bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT8810.12161050405552395Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATG3650.042.5294216
CGGTATT4350.042.40897824
ATGACGT3700.041.95469720
CGTCATA3700.041.95469724
TCGGTAT4400.041.92706323
GCGGTCT4450.041.45596736
TTCGGTA4450.041.45596722
AGCCGAT3750.041.395315
AAGCCGA3750.041.395314
CGATGAC3750.041.395318
TAAGCCG3750.041.395313
AGATTCG603.6379788E-1241.26525510
GGCGGTC4500.040.99534635
GATGACG3800.040.85062819
ATTCGGT4600.040.1041421
TCGGGGA4550.040.05039614
CGGGGAA4550.040.05039615
TCCTCGG4050.040.0147911
CCGATGA3900.039.80317317
AATTCGG4650.039.67291620
Produced by FastQC (version 0.11.5)