FastQCFastQC Report
Mon 6 Jun 2016
ERR1391302.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391302.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732028
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA39440.5387772052435152No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA28140.38441152524220384No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAACAATATCTCGTATGCCGTC24620.33632593288781304Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAACAATATCTCGTATGCC19950.27253055894036843Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT12180.16638707808990913Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATA4050.041.65964524
AGCCGAT4100.041.15160415
GCCGATG4100.040.60291716
GGCGGTC3550.040.55931535
ACGTCAT4200.040.17180323
GCGGTCT3600.039.9959936
TCGGGGA3600.039.9932614
AATTCGG3600.039.9932620
CGGTATT3600.039.9932624
TCGGTAT3600.039.9932623
TAAGCCG4250.039.69919213
GATGACG4200.039.63617719
TATGCCG3250.039.4548943
AGGCGGT3650.039.448134
TTCGGTA3650.039.44540822
GACGTCA4250.039.16987222
TGACGTC4250.039.16987221
ATGACGT4250.039.16987220
CCGATGA4300.038.7144117
CGATGAC4300.038.7144118
Produced by FastQC (version 0.11.5)