FastQCFastQC Report
Mon 6 Jun 2016
ERR1391301.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391301.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735528
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA39860.5419236249333812No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA27010.3672191949184803No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTGTGATCTCGTATGCCGTC26750.3636843192917197Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTGTGATCTCGTATGCC22860.3107971416451855Illumina Paired End PCR Primer 2 (97% over 34bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT12880.17511230027952707Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGTC3500.044.3537235
AGGCGGT3600.043.12167734
ATTCGGT3600.043.11874421
TTCGGTA3650.042.5280822
CGTCATA3850.042.0746824
AGCCGAT3850.042.0718215
GCGGTCT3700.041.95622636
CGGTATT3700.041.95622624
TCGGTAT3700.041.95622623
AATTCGG3700.041.95337320
AGATTCG650.041.5493810
GCCGATG3900.041.53243616
TGCCGTC3200.041.50657345
TATGCCG3200.041.47835543
TTCCTCG3150.041.43946510
TCGGGGA3750.041.39399714
GAATTCG3700.041.34535219
CTCGGGG3150.040.7083813
GACGTCA4050.040.54967522
CCGATGA4000.040.49412517