FastQCFastQC Report
Mon 6 Jun 2016
ERR1391299.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391299.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences576766
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA33730.5848125582992063No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA24160.41888738240464934No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGCGTAATCTCGTATGCCGTC9310.16141728187861282Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGCGTAATCTCGTATGCC7910.13714400640814473Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTCGG3250.042.918220
GAATTCG3250.042.918219
TCGGGGA3300.042.2679214
CGGGGAA3300.042.2679215
GCGGTCT3350.041.6370636
TTCGGTA3350.041.6370622
TCGGTAT3350.041.6370623
AAGCCGA3250.041.5337414
TAAGCCG3250.041.5337413
AGATTCG603.6379788E-1241.2596210
CCTCGGG2850.041.04796612
TTCGGGG556.002665E-1140.9044413
ATGACGT3250.040.84151520
TATGCCG1000.040.49539643
ATTCGGT3450.040.43018321
GGCGGTC3450.040.43018335
GCCGATG3350.040.29392616
CGGTATT3500.039.85606824
CTCGGGG2950.039.65650613
TGACGTC3350.039.6223621
Produced by FastQC (version 0.11.5)