FastQCFastQC Report
Mon 6 Jun 2016
ERR1391298.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391298.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657172
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA38540.5864522529870414No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA26310.4003518104849263No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGAGTCATCTCGTATGCC11610.17666607828696293Illumina Paired End PCR Primer 2 (97% over 34bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGAGTCATCTCGTATGCCGTC11410.17362273499175254Illumina Paired End PCR Primer 2 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAT4500.044.49360315
GCCGATG4600.043.52635216
ATGACGT4600.043.52635220
TAAGCCG4600.043.52304513
CCGATGA4650.043.05832717
CGATGAC4650.043.05832718
TCCTCGG2800.042.6220811
TGACGTC4700.042.60025821
GACGTCA4700.042.59701522
CGTCATA4750.042.1518424
TCGGGGA3650.041.90859614
AAGCCGA4800.041.7095814
CGGTATT3700.041.34540624
CCTCGGG2900.041.1116512
TATGCCG1150.041.0779343
ACGTCAT4900.040.86147323
TCGGTAT3750.040.79413223
TTCGGTA3750.040.7910322
ATTCGGT3700.040.73738521
GATGACG4950.040.4487319
Produced by FastQC (version 0.11.5)