ERR1391297.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1391297.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	799892
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA	4422	0.552824631325229	No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA	2833	0.3541728133297995	No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAGTAATCTCGTATGCCGTC	1963	0.24540813009756318	Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAGTAATCTCGTATGCC	1846	0.23078115545598654	Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT	858	0.10726448070489515	Illumina Paired End PCR Primer 2 (96% over 30bp)

Adapter graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTCATA	420	43.924664	24
GACGTCA	415	43.91176	22
AGCCGAT	435	43.444416	15
TGACGTC	430	42.903164	21
GCCGATG	430	42.903164	16
TAAGCCG	440	42.43941	13
CCGATGA	435	42.410023	17
ACGTCAT	435	42.410023	23
CGATGAC	430	42.379955	18
AAGCCGA	445	41.962563	14
ATGACGT	440	41.416775	20
TTCCTCG	280	40.99131	10
GATGACG	445	40.951416	19
TCGGTAT	375	40.79637	23
TTCGGTA	370	40.73962	22
ATTCGGT	375	40.196423	21
CGGTATT	375	40.196423	24
GCGGTCT	380	39.667522	36
GGCGGTC	380	39.667522	35
TCCTCGG	300	39.008724	11