FastQCFastQC Report
Mon 6 Jun 2016
ERR1391296.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391296.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847029
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA43820.5173376590411899No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA29110.34367182233430027No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGACTGATCTCGTATGCCGTC16490.19468046548583343Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGACTGATCTCGTATGCC14220.16788091080706802Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2000.042.74237443
GCCGATG4700.042.59877816
AGCCGAT4850.041.28129215
TCGGTAT3600.041.24507523
AATTCGG3600.041.2426420
CCGATGA4900.040.86005417
AAGCCGA4950.040.4473314
GCGGTCT3700.040.13034436
AGGCGGT3700.040.13034434
CGGTATT3700.040.13034424
TAAGCCG5000.040.04285413
CGTCATA5050.039.6487324
ATTCGGT3750.039.59293421
TTCGGTA3750.039.59293422
GAATTCG3750.039.59293419
CGATGAC5150.039.31337718
GGCGGTC3800.039.0742835
TGACGTC5200.038.9353621
ATGACGT5200.038.9353620
ATGCCGT2200.038.85670544
Produced by FastQC (version 0.11.5)