FastQCFastQC Report
Mon 6 Jun 2016
ERR1391295.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391295.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903769
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA51850.5737085472061998No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA36120.3996596475426796No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTGATGATCTCGTATGCCGTC19180.21222237098196553Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTGATGATCTCGTATGCC17790.1968423347116354Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGTC6600.042.2690921
GATGACG6600.042.2690919
CGATGAC6600.042.2690918
TAAGCCG6550.042.24593413
GACGTCA6650.041.95127522
GGCGGTC4350.041.8906335
CCGATGA6700.041.63820617
CGTCATA6700.041.63590624
GCCGATG6700.041.30241816
AGCCGAT6700.041.30013315
TTCGGTA4450.040.9515322
ACGTCAT6850.040.72416723
AAGCCGA6800.040.69277614
AGGCGGT4500.040.49427434
ATGACGT6900.040.43130520
GCGGTCT4550.040.04928236
CGGTCTC4550.040.04928237
CGGTATT4500.039.99434724
ATTCGGT4600.039.61615821
TCGGTAT4550.039.55484823
Produced by FastQC (version 0.11.5)