FastQCFastQC Report
Mon 6 Jun 2016
ERR1391293.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391293.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522003
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA37200.7126395825311349No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA25090.48064857864801547No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTGTGAATCTCGTATGCC14530.2783508907036933Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTGTGAATCTCGTATGCCGTC14360.27509420443943805Illumina Paired End PCR Primer 2 (96% over 32bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT6810.13045902035045776Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCAT4150.043.90799723
TAAGCCG4200.043.38528413
CGTCATA4200.043.38528424
ATGCCGT1950.042.68485344
GACGTCA4300.042.37632422
TGACGTC4300.042.37632421
GCCGATG4300.042.37632416
AAGCCGA4300.042.37632414
AATTCGG3150.042.135520
GCGGTCT3050.042.04184336
AGGCGGT3050.042.04184334
CCTCGGG2650.041.5965112
CTCGGGG2600.041.5312113
TTCGGTA3200.041.4771322
AGCCGAT4400.041.41322315
CCGATGA4400.041.41322317
GGCGGTC3100.041.36374335
CGATGAC4450.040.9479118
ATTCGGT3300.040.9019521
CGGGGAA3300.040.9019515
Produced by FastQC (version 0.11.5)