FastQCFastQC Report
Mon 6 Jun 2016
ERR1391285.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391285.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814946
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA55640.6827446235701506No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA36920.4530361520886046No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTAGCATCTCGTATGCCGTC16550.20308094033224286Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTAGCATCTCGTATGCC13660.16761846797211105Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG5600.044.18913713
CGTCATA5600.044.18913724
TGACGTC5750.043.03637721
GCCGATG5750.043.03637716
CGATGAC5750.043.03637718
GACGTCA5850.042.30071322
ACGTCAT5850.042.30071323
CCGATGA5900.041.9422317
ATGACGT5900.041.9422320
AGCCGAT5950.041.58977515
CGGGGAA5250.041.5645715
AGGCGGT5200.041.5316134
AATTCGG5200.041.5316120
TATGCCG1850.041.3445343
GGCGGTC5250.041.1360735
GCGGTCT5300.040.7479936
ATTCGGT5300.040.7479921
GATGACG6100.040.56707819
GAATTCG5400.040.40999619
TCGGGGA5450.040.03926514
Produced by FastQC (version 0.11.5)