FastQCFastQC Report
Mon 6 Jun 2016
ERR1391283.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391283.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882095
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA47180.5348630249576293No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA33390.3785306571287673No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCGAGAATCTCGTATGCCGTC15850.17968586150017854Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCGAGAATCTCGTATGCC13790.15633236782886195Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATA5100.043.22946524
TAAGCCG5150.042.80976513
ACGTCAT5250.042.4228523
AGCCGAT5250.041.9943415
CCGATGA5300.041.59816717
CGATGAC5400.041.2444418
AATTCGG4150.041.19926520
GACGTCA5400.040.82782722
TGACGTC5450.040.45326221
AAGCCGA5450.040.45326214
ATTCGGT4300.040.28526721
TTCGGTA4250.040.2298722
ATGACGT5500.040.08550620
TATGCCG1750.039.8517743
GCGGTCT4300.039.7620836
AGGCGGT4250.039.7005334
TCGGGGA4350.039.30504614
TCGGTAT4350.039.30504623
GATGACG5650.039.0212919
GGCGGTC4450.038.42178735
Produced by FastQC (version 0.11.5)