FastQCFastQC Report
Mon 6 Jun 2016
ERR1391278.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391278.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1404390
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA41780.29749570988115837No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA30270.21553841881528638No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCTCTCTATCTCGTATGCCGTC19650.13991839873539402Illumina Paired End PCR Primer 2 (97% over 35bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTCTCTATCTCGTATGCC14150.10075548814787916Illumina Paired End PCR Primer 2 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTCT3400.041.6866936
AGCCGAT4900.041.32061415
TCGGTAT3550.040.55901323
AATTCGG3500.040.49564420
GCCGATG5000.040.4956416
GGCGGTC3500.039.85285635
TTCGGTA3650.039.4478122
ACGTCAT5250.039.42432823
CGATGAC5250.039.42432818
GACGTCA5200.039.3707622
CGGTATT3600.039.3707624
GAATTCG3600.039.3707619
CGTCATA5200.039.3707624
CCGATGA5250.038.995817
GATGACG5250.038.995819
TAAGCCG5200.038.9367313
TGACGTC5350.038.2669121
ATGACGT5350.038.2669120
AAGCCGA5300.038.20207614
TGCCGTC2100.037.50798445
Produced by FastQC (version 0.11.5)