FastQCFastQC Report
Mon 6 Jun 2016
ERR1391276.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391276.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659205
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA47330.7179860589649654No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA32840.4981758330109753No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTAGCAATCTCGTATGCCGTC22540.34192701815065113Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTAGCAATCTCGTATGCC19860.3012719867112658Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT10780.1635303130285723Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG5750.043.4282713
AGCCGAT5800.043.0538915
GCCGATG5800.042.66601616
GATGACG5800.042.66601619
GACGTCA5850.042.30134622
ACGTCAT5850.042.30134623
CGATGAC5850.042.30134618
ATGACGT5900.041.94285620
GCGGTCT4100.041.7010636
AATTCGG4100.041.7010620
TCGGTAT4100.041.7010623
TGACGTC5950.041.590421
CGTCATA5950.041.590424
AAGCCGA6050.041.274814
CCGATGA6000.041.24381317
GGCGGTC4150.041.1986435
TTCGGTA4150.041.1986422
CGGTATT4150.041.1986424
ATTCGGT4300.039.7614821
CGGGGAA4300.039.7614815
Produced by FastQC (version 0.11.5)