FastQCFastQC Report
Mon 6 Jun 2016
ERR1391275.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391275.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466491
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA50311.0784773982777802No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA32320.6928322304181646No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATAGCGATCTCGTATGCCGTC14180.3039715664396526Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATAGCGATCTCGTATGCC10700.22937205648126116Illumina Paired End PCR Primer 2 (97% over 35bp)
TCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA7090.1519857832198263No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT6490.13912379874424158Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATA4750.044.52175524
TAAGCCG4800.044.05798713
ACGTCAT4900.043.6179823
GGCGGTC4650.043.54392635
AGCCGAT4900.043.15884415
CCGATGA4900.043.15884417
GACGTCA4900.043.15884422
AATTCGG4700.043.08069220
TCGGTAT4700.043.08069223
TGACGTC4950.042.72289721
GCCGATG4950.042.72289716
AGGCGGT4800.042.65188234
ATTCGGT4750.042.62721321
CGGTATT4750.042.62721324
AGATTCG900.042.51843610
CGATGAC5000.042.2956718
TTCGGTA4800.042.18317822
TCCTCGG3900.042.1336511
AAGCCGA5050.041.87689614
ATGACGT5050.041.87689620