FastQCFastQC Report
Mon 6 Jun 2016
ERR1391271.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391271.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences639667
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA41910.6551846507636004No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA28850.45101591922046935No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGAGTACATCTCGTATGCCGTC14450.22589878796311205Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGAGTACATCTCGTATGCC11570.1808753617116406Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT8280.12944235047298047Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATA4850.043.59991524
GGCGGTC4550.043.0137635
TCGGTAT4550.043.0137623
AAGCCGA4950.042.7191114
ACGTCAT4950.042.7191123
TAAGCCG4950.042.7191113
AGGCGGT4550.042.51935234
CGGTATT4550.042.51935224
AGCCGAT5000.042.29191615
GCCGATG5000.042.29191616
GATGACG5000.042.29191619
GCGGTCT4650.042.0887336
AATTCGG4650.042.0887320
TATGCCG1450.041.88854643
TGACGTC5050.041.87318421
CGGGGAA4700.041.64097615
TTCGGTA4700.041.64097622
ATTCGGT4650.041.6049521
AGATTCG650.041.56301510
CCGATGA5150.041.49692517